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Polymerase chain reaction (PCR) to identify:

  • Chytrid fungus (Batrachochytrium dendrobatidis)
  • Bacterial kidney disease bacterium (Reninbacterium salomoninarum)
  • Didymo diatom algae (Didymosphenia geminata)
  • Whirling disease bacterium (Myxobolus cerebralis)
  • Microsporidial Gill Disease parasite (Loma salmonae)
  • Eurasian watermilfoil (Myriophyllum spicatum)
  • Quagga mussel (Dreissena rostriformis) & Zebra mussel (Dreissena rostriformis)
  • Ranavirus

 

Amplified fragment length polymorphism (AFLP) analysis of cutthroat trout subpecies (Oncorhynchus clarkii), including greenback, Rio Grande, Colorado River, Yellowstone, and rainbow cutthroat trout lineages

 

DNA analysis: Including reanalysis, extraction, genetic purity and/or introgression, mitochondrial NDE2 and CO1 sequencing, quality and quantity, lack of PCR inhibition, microsatellite markers

 

Sampling kits:  We offer field-ready swab sample collection kits for both individual samples and pooled samples. Using sample collection kits eliminates the need to locate and order the required tubes, ethanol, swabs and distilled water, and the labor of cutting swabs and dispensing alcohol. The kits have been used for thousands of swab samples. They are optimal for leak-free shipping and efficient sample processing.The tubes in the kit contain pre-measured 70% ethanol made with histological grade reagent alcohol and ultrapure deionized water. The swabs have been pre-cut to a length that will not interfere with proper cap tightening. The tubes will not leak during shipping if the caps are threaded and tightened correctly.

 

Archiving: We archive all sample DNA preparations indefinitely, providing for future genetic and disease testing

 

Pooling: We offer testing of “pooled” swab samples – combining the swabs taken from multiple individual animals into one DNA preparation and one PCR test. This allows the screening of large numbers of animals across large populations or geographic areas at greatly reduced cost.

 

Consulting & Presentations : Please call for more information. Previous work has included eDNA and PCR assay development, trainings in DNA and PCR detection methodologies, molecular genetics procedures for SMRT, aquatic nuiscance species identification, and quantitative PCR using a positive plasmid control.

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